![]() Now you create a statistic that tells you the median forward scatter signal for "Live cells." Thus, you create a new node (analysis) attached to the "Live cells" gate of the first sample. After looking at the 3 samples, youįind that you have to alter the position of the gate slightly for each one-therefore, the gates are no longer identical. ![]() You first decide on a gate to exclude dead cells and clumps of cells you apply this gate to a group containing all three samples (and call it "Live cells"). During batch operations, you can specify that this analysis be applied to all samples within a group.įlowJo decides how to do this by looking at the names of the gates.įor instance, you may have 3 samples in a workspace. When you specify a kind of analysis (for instance, a statistical measurement, or a gate selecting a subset of cells), it is applied to a population. Gates are similar to what we expect for rules for naming people in families: siblings cannot have the same name however, different generations can have gates with the same name. ![]() Naming populations is very important for the way in which FlowJo works. FlowJo identifies populations (samples, or gated subsets of samples) by a name that you assign.
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